Table 5 Primers, corresponding references and PCR cycles used to Cyt b, 16S rRNA, D-loop and RAG-2

Cyt b

L14724

[55]

[30 s at 94 °C, 30 s at 52 °C, 70 s at 72 °C] × 35

H15915

16S rRNA

16Sp1F

[56]

[30 s at 94 °C, 30 s at 48 °C, 90 s at 72 °C] × 33

16Sp1R

D-loop

GEDL200

[57]

[30 s at 94 °C, 30 s at 52 °C, 60 s at 72 °C] × 35

GEDH860

RAG-2

RAG2-f2

[58]

[60 s at 94 °C, 60 s at 60 °C, 105 s at 72 °C] × 35

RAG2-R6