Fig. 4

Metabolite turnover fluxes in glycolysis and TCA cycle. Fluxes in the glucose specialist (a, b) and the acetate specialist (c, d) genotypes (model IDs 12364 and 44490, respectively) during growth on glucose (a, c) and acetate (b, d). The following metabolites and reactions are shown: ac, acetate; actp, acetyl-phosphate; akg, alpha-keto-glutarate; cit, citrate; f6p, fructose-6-phosphate; fum, fumarate; glx, glyoxylate; g6p, glucose-6-phosphate; icit, isocitrate; mal, malate; oaa, oxaloacetate; pep, phospho-enol-pyruvate; succ, succinate; succoa, succinyl-coenzyme a. PGI, ACN, ACE, and ACK are the reactions catalyzed by glucose-phosphate isomerase, aconitate hydratase, malate synthase, and acetate kinase, respectively (shown in blue). Thickness of the arrow indicates the flux over the given reaction; the reference arrow at the bottom right shows a flux of 10 mmol/gDW/h