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Fig. 1 | BMC Evolutionary Biology

Fig. 1

From: Evolution of RLSB, a nuclear-encoded S1 domain RNA binding protein associated with post-transcriptional regulation of plastid-encoded rbcL mRNA in vascular plants

Fig. 1

RLSB homologs are present and conserved across a broad range of plants species. Arabidopsis and maize RLSB family transcripts were used as reference sequences to search for orthologs in a broad assortment of plant species representing major plant groups, ranging from algae to angiosperms. Representative species included in the alignment shown here are Chara vulgaris and Saragassum thunbergi representing algae, Marchantia palaeceae, Sphagnum recurvum representing liverworts and mosses respectively, Sellaginella sellaginoides, a lycophyte, Pinus ponderosa, a gymnosperm, Amborella trichopoda, representing basal angiosperms, Ascarina rubricaulis, belonging to the order Chloranthales of Angiosperms, Magnolia grandiflora, representing magnolids, Papaver somniferum, an early eudicot, Boswellia sacra and Flaveria bidentis represent the core eudicots, and Agave tequiliana is a monocot that also utilizes CAM photosynthesis. The BLAST search using the tblastn algorithm in the 1000 Plants database (https://www.bioinfodata.org/Blast4OneKP/blast) revealed scaffolds in various plant species with sequence similarity to RLSB family members. Multiple sequence alignment using T-coffee sequence aligner software (http://tcoffee.crg.cat/apps/tcoffee/index.html) shows the most conserved regions within the homologs, including the S-1 binding domain region. TCS analysis of the alignment [18] showed a score higher than 85 for all species, indicating a highly reliable alignment. Note that less conserved regions at the N-terminal and C-terminal areas are not shown in this figure

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