Fig. 2

Expression of Wnt genes in Halisarca dujardini. a schematic representation of the adult body plan of Halisarca, apical and basal regions and the osculum are labelled; b, c, HduWntD and HduWntE transcripts are localized around the osculum; d, HduWntG transcripts are present throughout the exopinacoderm and particularly in the oscular chimney; e, f, f’, HduWntF and HduWntH transcripts are absent from the osculum and the apical region, but strong along the base; g, HduWntK transcripts are present along the oscular chimney; h and i, HduWntJ transcripts are present in the oocytes; j, HduWntK transcripts are present in the posterior half of the larva except the polar region; l, HduWntJ transcripts are present in cells distributed along the larval equator; l and m, HduWntK transcripts are conspicuously present along the wound margin. White arrowheads indicate the osculum; insets in the upper and lower corners are enlargements of the apical and basal regions, respectively, black arrowheads indicate wound margin; black arrows indicate oocytes; posterior pole of the larvae is towards the top. Note that the specific staining is dark purple, while the uniform pink coloration of some samples is background staining. Scale bars: b, d, e – 5 mm; c – 2.5 mm; f, f’ – 2 mm; g, h, l, m – 3 mm; i – 30 μm; j, k – 50 μm