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Table 1 Summary of tests for phenotypic differences related to experimental evolution under high promiscuity

From: Experimental evolution under hyper-promiscuity in Drosophila melanogaster

Phenotype

Male type

Female type

Generation tested in evolved flies

Behavioural phenotypes

 Mating frequency in population cages

SPR-, C

SPR-, C

1–17, 28

 Courtship frequency

SPR-, C

WT

16, 26

 Latency to mating

SPR-, C

WT

16, 36

 

WT

SPR-, C

26

 

SPR-, C

SPR-, C

26

 Copulation duration

SPR-, C

WT

16, 36

 

WT

SPR-, C

26

 

SPR-, C

SPR-, C

26

 Female post-mating fecundity; for males, ability to stimulate fecundity

SPR-, C

WT

16

 

WT

SPR-, C

26

 Female latency to re-mating; for males, ability to inhibit re-mating

SPR-, C

WT

16, 36

 

WT

SPR-, C

26

 

SPR-, C

SPR-, C

26

 Sperm precedence as first male to mate with a female (P1)

SPR-, C

WT

36

 Sperm precedence as second male to mate with a female (P2)

SPR-, C

WT

36

Morphological evolution

 Male mass

SPR-, C

–

16

 Testes area

SPR-, C

–

16

 Accessory gland area

SPR-, C

–

16

Gene expression

  SP gene expression

SPR-, C

–

55

  Dup99B gene expression

SPR-, C

–

55

  1. Phenotypes tested for differences between sex peptide receptor deficient (SPR-) flies and controls that expressed SPR (C). We tested experimental males with wild-type females, experimental females with wild-type males, or within-treatment tests of SPR- or control males and females paired within replicate line