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Table 2 Fossil calibration used for calibrating the shark phylogeny. Minimum and upper bound fossils with estimated ages and references for nodes 1, 2, 3, 5 and 8

From: Evidence of positive selection associated with placental loss in tiger sharks

Calibration Point

Fossil (minimum age)

Lower Bound Fossil Age (mya)

Reference

Fossil (maximum age)

Upper Bound Fossil Age (mya)

Reference

Lamniformes – Carcharhiniformes

(Node 1)

Paleoscyllium tenuidens

169.6

Underwood & Ward, 2004 [31]

Paracestracion

183.4

Delsate & Lepage, 1990 [28]

Triakidae – Carcharhinidae

(Node 2)

Carcharhiniformes Fossil

132.9

Underwood et al. 1999 [30]

Paleoscyllium tenuidens

169.6

Underwood & Ward, 2004 [31]

Galeocerdo – Carcharhinidae

(Node 3)

Galeocerdo latidens

47.8

Noubhani & Cappetta, 1997 [29]

Carcharhiniformes Fossil

132.9

Underwood et al. 1999 [30]

NegaprionCarcharhinus/Prionace (Node 5)

Aprionodon acutidens

16.0

Probst, 1879 [26]

Galeocerdo latidens

47.8

Noubhani & Cappetta, 1997 [29]

Prionace – Carcharhinus perezii (Node 8)

Squalus glauca

3.6

Landini, 1977 [27]

Aprionodon acutidens

16.0

Probst, 1879 [26]