Table 3 Reference transcriptome NvecRef32743 statistics

Total number of raw reads pooled for de novo assembly

137,142,632

Assembled “transcripts”

32,743

Transcripts remaining after alien index analysis

32,706

Transcripts encoding an ORF > 100aa in length

27,511

Transcripts with non-0 expression and ORF >100aa

17,313

New transcripts (not predicted from the genome)

2,150

Mean transcript length in bp (N = 32,706/17,313)

1,058/1,646

N50 in bp (N = 32,706/17,313)

1,919/2,282

GC content (N = 32,706/17,313)

44.56 %/45.5 %