Do novel genes drive morphological novelty? An investigation of the nematosomes in the sea anemone Nematostella vectensis

Table 2 Error correction increases the mapping concordance for transcriptome NvecRef32743

Sample^a	Raw reads (paired)	Reads remaining after trimming^b	Reads remaining after trimming & Error Correction^c	Reads aligned^d – Trimmed only	Reads aligned – Trimmed & Error Corrected
M3	49,390,203	49,377,414	46,888,223	39,768,569 (81 %)	43,249,696 (92 %)
M4	47,477,373	47,466,721	44,704,490	36,255,081 (76 %)	39,339,951 (88 %)
Mes	26,632,325	26,615,206	25,785,788	24,435,421 (92 %)	24,380,462 (95 %)
N1	47,788,007	47,770,601	44,558,275	36,611,388 (77 %)	39,656,864 (89 %)
N2	53,500,454	53,483,271	50,693,980	42,513,852 (79 %)	45,609,374 (90 %)
Nem	20,135,090	20,073,479	19,174,413	17,995,873 (90 %)	17,834,121 (93 %)
T3	47,683,996	47,670,541	44,585,027	27,963,673 (77 %)	39,854,556 (89 %)
T4	50,314,893	50,300,293	47,474,608	40,154,724 (80 %)	43,557,953 (92 %)
Ten	21,803,901	21,788,366	20,983,324	19,474,441 (89 %)	19,384,395 (92 %)

^aSamples M3, M4, Mes are from mesenteries; N1, N2, Nem are from nematosomes; T3, T4, Ten are from tentacles. ^bTrimming was performed using Trimmomatic [52]. ^cError correction was performed using the ErrorCorrectReads utility from AllPaths-LG [53]. ^dAlignments were performed using Bowtie2 [54]

ISSN: 2730-7182