R-gene variation across Arabidopsis lyrata subspecies: effects of population structure, selection and mating system

Table 2 Observed heterozygosity and nucleotide diversity across European A. l. petraea and N. American A. l. lyrata outcrossing samples at 6721 RAD loci, RPM1 and WRR4

	RAD loci			RPM1					WRR4
Group	N	H _o	π	N	H _o	π	π_syn	π_non-syn	N	H _o	π	π_syn	π_nonpsyn
Sweden	6	0.149	0.0025	6	0.500	0.0029	0.0038	0.0027	6	0.500	0.0007	0.0025	0.0002
Norway	7	0.154	0.0028	7	0.429	0.0021	0.0031	0.0018	7	0.429	0.0026	0.0069	0.0013
Iceland	3	0.132	0.0020	4	0.250	0.0003	0.0000	0.0003	4	0.500	0.0031	0.0089	0.0014
Scotland	2	0.155	0.0024	9	0.333	0.0009	0.0012	0.0008	11	0.273	0.0036	0.0100	0.0017
Austria	2	0.195	0.0033	9	0.889	0.0022	0.0051	0.0014	8	0.375	0.0055	0.0149	0.0028
Germany	3	0.223	0.0031	5	1.000	0.0021	0.0023	0.0020	3	0.000	0.0047	0.0104	0.0030
European average	23	0.162	0.0039	40	0.575	0.0028	0.0047	0.0022	39	0.359	0.0041	0.0108	0.0022
N. American Great Lakes outcrossing	28	0.074	0.0015	66	0.530	0.0019	0.0040	0.0013	66	0.576	0.0022	0.0056	0.0012

The samples size (N) for RAD-seq included 28 Great Lakes outcrossing individuals from seven populations and for both R-genes included 66 Great Lakes outcrossing individuals from 11 populations included (Additional file 1: Table S2 has details of sample locations). Observed heterozygosity (H_o): Average proportion of heterozygous loci per individuals (RAD-seq) or proportion heterozygous individuals (R-genes). Nucleotide diversity (π, as calculated by [47]). For the RAD loci this is the average number of pairwise differences across 6721 loci divided by the length of the RAD sequence (92 bp). Synonymous/non-synonymous nucleotide diversity (π_syn/non-syn) calculated using DnaSP version 5 [48]

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