Fig. 2From: Contrasting effects of historical contingency on phenotypic and genomic trajectories during a two-step evolution experiment with bacteriaTwo-step experimental evolution design. Populations were initiated from a single ancestor (Anc) and propagated in four initial historical environments named Ace (Acetate), Gly (Glycerol), Glc (Gluconate), and Glu (Glucose) during 1000 generations (phase I) [22]. From each replicate population, both a population sample and a randomly isolated evolved clone were preserved and used both for phenotypic assays and to found new populations that were propagated in a single new environment during 1000 generations (phase II). At the end of phase II, a population sample from each of the populations as well as a randomly isolated evolved clone from each of the populations founded from the individual evolved clones derived from phase I were preserved. Genome sequences from the evolved clones sampled after phase I have been previously determined [22] and here we sequenced the genomes the evolved clones sampled after phase II (see Methods)Back to article page