Fig. 9

Brain organization of females of Osedax spp., Confocal laser scanning microscopy (CLSM) and light microscopy (LM). a-c Frontal CLSM optical sections of the brain of O. “nudepalp E” from the most dorsal in (a) to the most ventral part in (c) (cyan, acetylated α-tubulin IR; red, serotonin LIR). d-g Frontal CLSM optical sections of the right side of the brain of O. mucofloris, from the most ventral in (d) to the most dorsal side of the brain in (g) (cyan, acetylated α-tubulin IR). h Brain and associated neural elements of O. priapus (red, serotonin LIR). i Frontal CLSM optical section through the median part of O. priapus (cyan, acetylated α-tubulin IR). g-l. Transverse histological sections of the anterior trunk in the area of the brain, from the middle part of the brain in (j) to the anterior part of the brain in (l). m LM precise view of the anterior part of the brain showing the perikarya concentration. Abbreviations: acbr – anterior commissure of the brain, adn – antero-dorsal nerve, ann – anterior nerve net, br – brain, brp – brain perikarya, cnn – circular nerve net, col – collar, cut – cuticular shield, cvc – commissure of the ventral cords, dln – dorso-lateral nerve bundle, dlp – dorsal left palp, dlpn2 – dorsal left palp nerve 2, drpn1, 2 – dorsal right palp nerve 1, 2, lln – lateral longitudinal neurite bundles in the brain, mcc – middle cross commissure, msc – middle serotonergic commissure, ovd – oviduct, pcbr – posterior commissure of the brain, pcnn – perikarya of the cnn, pvc – perikarya of the vcrn – root nerve, trc – trunk coelom, vc – ventral cord, vcp – ventral cord projection, vln – ventro-lateral nerve, vlp – ventral left palp, vpann – ventral perikarya of ann, vrpn 2 - ventral right palp nerve 2, vrp – ventral right palp