Fig. 5

Confocal laser scanning microscopy of female Osedax mucofloris. Depth coded images (in a-c, g) indicate the depth of the signal in the Z-stack: scale follows the colors of the spectral light, the top is to the surface. a Dorsal view of anterior region, total depth of Z-stack (depth coded colors, acetylated α-tubulin IR). b Dorsal view of the dorsalmost part of Z-stack only (depth coded colors, acetylated α-tubulin IR). c Ventral view of the anterior region showing brain (depth coded colors, acetylated α-tubulin IR). d Ventro-lateral view of the anterior region, the lateralmost part of Z-stack only (red, serotonin LIR; blue, acetylated α-tubulin IR). e Lateral view of anterior region (red, serotonin LIR; blue, acetylated α-tubulin IR). The black box in the upper left corner is an artifact of the stiching of two images that are not overlapping in this area. f Cross section of trunk (green, phalloidin; blue, acetylated α-tubulin IR; red, serotonin LIR). g Close up of the ventral part of (f) showing glands (depth coded colors, acetylated α-tubulin IR). h Close up of glands from (g) (green, phalloidin; blue, acetylated α-tubulin IR; red, serotonin LIR). Abbreviations: and – antero-dorsal nerve, ann– anterior nerve net, aon – anterior oviduct nerve, br – brain, cb – ciliary band, cm – circular muscles, cnn – circular nerve net, ct – ciliary tuft, cvc – commissure of the vc, dbv – dorsal blood vessel, ddn – dorso-diagonal nerve, dn – dorsal nerve, dln – dorso-lateral nerve bundle, dln1-5 – dorso-lateral nerves 1-5, dlpn1,2 – dorsal left palp nerve 1, 2, drpn1, 2 – dorsal right palp nerve 1, 2, gc – glandular cell, gn – glandular cell neck, mn – median nerve, vbv – ventral blood vessel, vc – ventral cord, vcp – ventral cord projection, vcpp – posterior process of the vc, vln – ventro-lateral nerve, vlpn1, 2 – ventral left palp nerve 1, 2, vrpn1, 2 - ventral right palp nerve 1, 2