Fig. 4

Confocal laser scanning microscopy of female Osedax mucofloris ( a , b , e - i ) and O. yellow collar (c, d) Depth coded images (in b, f, h) indicate the depth of the signal in the Z-stack: scale follows the colors of the spectral light, the top is to the surface. a Whole specimen (red, FMRFamide LIR; blue and cyan, acetylated α-tubulin IR), darker patch posteriorly to brain is an artifact of the labelling procedure. b Lateral view of anterior region (depth coded colors, FMRFamide LIR). c Lateral view of anterior region of trunk (red, FMRFamide LIR; blue, anti α-tubulin IR). d Whole specimen (red, anti serotonin LIR; blue, anti acetylated α-tubulin IR). e Ventral side of the posterior trunk (pink, serotonin LIR; blue, acetylated α-tubulin IR). f Lateral view of the posterior trunk (depth coded colors, acetylated α-tubulin IR). g Dorsal view of the posterior trunk (pink, serotonin LIR; blue, acetylated α-tubulin IR; green, phalloidin). h Dorsal view of the posterior trunk (depth coded colors, acetylated α-tubulin IR). i Ventral view of the anterior trunk showing straight and crossing neurite bundles of the ventral cords. Abbreviations: aon – anterior oviduct nerve, br – brain, cb – ciliary band, cnn – circular nerve net, ctn – circular trunk neurites, cvc – commissure of the vc, cvn – crossing neurites of the vc, dn – dorsal nerve, dln – dorso-lateral nerve bundle, dlpn1,2 – dorsal left palp nerve 1, 2, dpnn – dorsal posterior nerve net, drpn 2 – dorsal right palp nerve 2, mn – median nerve, mvn - median ventral nerve, oln – ovisac longitudinal nerves, os – ovisac, pcb – palp ciliary band, pp – palp pinules, ron – ring ovisac nerve, svn – straight ventral neurites of the vc, tpl – trunk plexus, tr – trunk, vc – ventral cord, vcp – ventral cord projection, vcpp – posterior process of the vc, vln – ventro-lateral nerve, vlp 2 – ventral left palp nerve 2, vpnn – ventral posterior nerve net