Using a multilocus phylogeny to test morphology-based classifications of Polystichum (Dryopteridaceae), one of the largest fern genera

Table 3 Selected molecular markers and their primers used in this study

Molecular marker	Primer name	Sequence (5′➔3′)	References
psbA-trnH	psbA	GTT ATG CAT GAA CGT AAT GCT C	[85]
	trnH	CGC GCA TGG TGG ATT CAC AAT CC	[86]
rbcL	rbcL-1F	ATGTCACCACAAACAGAAACTAAAGC	[87]
	rbcL-595F	AAT TCY CAR CCR TTC ATG CGT	This study
	rbcL-895R	AGC TAA GCT GGT RTT KGC RGT	This study
	rbcL-1375R	TCACAAGCAGCAGCTAGTTCAGGACTC	[88]
rps4-trnS	trnS	ATG AAT T(A/G)T TA G TTG TTG AG	[89]
	rps4	TAC CGA GGG TTC GAA TC	[90]
trnL-trnL-trnF	fern1	GGCAGCCCCCARATTCAGGGRAACC	[91]
	F	ATTTGAACTGGTGACACGAG	[92]

ISSN: 2730-7182