Fig. 2

Deletion analysis of the Bf-Gsx-Up-Proximal construct. a Deletion map showing the deletion analysis of the Bf-Gsx-Up-Proximal construct with the numbers of embryos exhibiting LacZ expression in the nerve cord only, sensory vesicle only, or nerve cord with sensory vesicle for each construct recorded both as a percentage of the total number of embryos that developed, and as raw numbers of embryos expressing LacZ for each domain alongside the total number of embryos that developed. Grey regions indicate the relative positions of each construct compared to the Bf-Gsx-Up-Proximal construct, with 5′ and 3′ limits denoted in number of base pairs from the B.floridae Gsx translational start site. Blue regions denote coding sequence, whereas orange regions indicate the pCES Forkhead promoter. The Grey dashed-arrow indicates that the pCES Forkhead-LacZ construct directly abuts the displayed regulatory region in each reporter construct. b Lateral view of a mid tailbud Ciona embryo displaying ‘nerve cord only’ LacZ expression. c Lateral view of a mid tailbud Ciona embryo displaying ‘sensory vesicle only’ LacZ expression. d Lateral view of a mid tailbud Ciona embryo displaying the full ‘nerve cord with sensory vesicle’ LacZ expression pattern. e Dorsal view of a mid tailbud Ciona embryo displaying ‘nerve cord only’ LacZ expression. f Dorsal view of a mid tailbud Ciona embryo displaying ‘sensory vesicle only’ LacZ expression. g Dorsal view of a mid tailbud Ciona embryo displaying the ‘nerve cord with sensory vesicle’ LacZ expression pattern. Black arrows denote LacZ expression within the nerve cord. Black arrowheads denote LacZ expression in the sensory vesicle