|  | %GC | % lone NQ †| % run NQ †| % lone N †| % lone Q †| % run N †| % run Q †|
|---|
Trend clades** | −0.14 (NS) | 0.22 (NS) | 0.84 (0.00016) | 0.18 (NS) | −0.11 (NS) | 0.41 (NS) | 0.70 (0.0037) |
Saccharomycetes | −0.68 (0.00014) | 0.42 (0.0054) | 0.73 (<0.00001) | 0.51 (0.0007) | −0.39 (0.009) | 0.74 (<0.00001) | 0.21 (NS) |
Onygenales | 0.54 (NS) | 0.03 (NS) | 0.02 (NS) | −0.09 (NS) | 0.07 (NS) | −0.11 (NS) | 0.15 (NS) |
Eurotiales | 0.27 (NS) | −0.33 (NS) | −0.21 (NS) | −0.37 (NS) | 0.20 (NS) | −0.33 (NS) | −0.04 (NS) |
Eurotiomycetes *** | 0.16 (NS) | −0.24 (NS) | 0.30 (NS) | 0.20 (NS) | 0.60 (0.0005) | 0.22 (NS) | 0.37 (0.03) |
- *Spearman rank correlation coefficients (with P-values) for one-tailed test, for the trend clades; Pearson correlation coefficients for the other analysis. The two most significant for each row are in bold
- **The trend clades are the obvious groupings from examining the trend across Ascomycota and Basidiomycota for %NQPs in Additional file 3: Figure S1, and depicted in schematic Fig. 3. No significant results are found by simply considering the six subphyla (three from Ascomycota, three from Basidiomycota)
- ***Contains both the Onygenales and Eurotiomycetes and a small number of other organisms
-
†‘Lone’ N and Q do not occur in homopeptide runs and are surrounded on either side by ≥2 non-N/Q residues. ‘Run’ N and Q occur in runs of 3–5 residues. Both ‘lone’ and ‘run’ N and Q residues are counted only from proteins that are non-N/Q-biased proteins (using a strict P-value threshold of <1×10−5), and that are not predicted to be prions by PAPA or PLAAC. ‘Lone NQ’ and ‘run NQ’ are simply the sums of ‘lone N’ and ‘lone Q’, and ‘run N’ and ‘run Q’ respectively
