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Fig. 6 | BMC Evolutionary Biology

Fig. 6

From: The evolution of heat shock protein sequences, cis-regulatory elements, and expression profiles in the eusocial Hymenoptera

Fig. 6

Relative basal heat shock gene (target) expression (+/− SD) between P. barbatus(N = 3) and A. picea (N = 4). Relative gene expression was normalized with the geometric mean of 18 s rRNA and β-actin as the calibrator (* = p < 0.05;** = p < 0.01; *** = p < 0.001 levels of significance) and fold change was calculated as P. barbatus relative to A. picea was calculated as follows: 2Target(Pbar-Apic)/2Calibrator(Pbar-Apic)(Pbar = P. barbatus, Apic = A. picea). -1 was divided by values less than one to calculate negative relative basal expression. Significant up-regulation in P. barbatus and A. picea are colored in red and blue, respectively

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