Fig. 2

Alignment of the conserved motifs of the glycoside hydrolase 32 (GH32) enzymes. These include conserved regions (labelled A-G) and various amino acids (shown with black stars). The N-terminal β-propeller module (indicated in the blue block) and the C-terminal β-sandwich module (indicated in the red block) are also highlighted. The translated sequences of one group of the Ceratocystis GH32 gene possess a trans-membrane domain (shown with dotted lines) characteristic of vacuolar invertases [5], while the translated sequences of the other Ceratocystis GH32 gene and the Huntiella GH32 gene possess an eukaryotic secretion signal (shown with dotted lines) needed for secretion [60]