Table 1 Reference gene and gene sequences encoding immune and antioxidant enzymes used in qPCR
Genes | Sequences (5’-3’) | GenBank |
|---|---|---|
Lysozyme | F-TATTCCACGCGACCAGTTGG | KT358925 |
R-GGCGAGTCAGATTTGGAGCA | ||
Acid phosphatase | F-TACCGGAATGGACCCGTATG | KT363040 |
R-TCGTAGAACCGTCCCAAGGA | ||
Pro-phenol oxidase | F-AAGGAGGAGACGAACTGGCC | KT363041 |
R-CAATTGGCATGCAGTTCCTG | ||
Carboxylesterase | F-TCAAGGCCAGCTTCTGATGA | KT363042 |
R-GAAGAAGTGGATCCGCGTCT | ||
Peroxidase | F-CAGCGAATCCCAGTTGGATG | KT363043 |
R-TGGCTTCGAACACACGAGGT | ||
HSP60 | F-GCCGTCGAGGAAGGTATCGT | KT363038 |
R-ACACCGTTTGCTTGATCCGA | ||
Tubulin beta | F-CTCCGACGAACATGGAATCG | ADI24738.1 |
R-TTGCCACCAGACGCTTCATT |