Fig. 6

Expression patterns of OMP1 and 2 at the cellular level in the OE. Two-color fluorescence in situ hybridization analysis using DIG- or fluorescein-labeled antisense riboprobes in horizontal sections of the adult zebrafish OE. a, d, g Fluorescent images of Alexa 594 derived from DIG-labeled riboprobes. b, e, h Fluorescent images of Alexa 488 derived from fluorescein-labeled riboprobes. c, f, i Merged images of (a) and (b), (d) and (e), and (g) and (h), respectively. Yellow two-headed arrows and cyan two-headed arrows indicate the superficial layer and the deep layer, respectively. Dashed lines indicate the outlines of the epithelium. White arrowheads indicate cells that coexpress OMP2 and another gene. Scale bar, 20 μm