Fig. 5

Expression patterns of OMP1 and 2 at the tissue level. a RT-PCR analysis of OMP1, OMP2, and GAPDH (positive control) in adult zebrafish. DNA templates were as follows: B, brain; OE, olfactory epithelium; E, eye; G, gill; S, skin; T, testis; O, ovary; Gen, genomic DNA. RT–, the cDNA synthesis was performed without reverse transcriptase as a negative control for RT-PCR of OMP1. Note: When genomic DNA was used as a tmplates, PCR using OMP2 or GAPDH primers did not amplified fragments because of the presence of introns (over 2 kb) in the corresponding sequences in the genomic DNA. b Expression of OMP genes in eyes of gar and zebrafish. Bars indicate FPKM ratio of each OMP to Gαt1, which is coupling with rhodopsins. The number of spotted gar indicate technical replicate. c, d Fluorescence in situ hybridization analysis for OMP2 using DIG-labeled antisense riboprobes (c) or sense riboprobes (d) in transverse sections of adult zebrafish eyes, which were counterstained with DAPI. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Arrow indicates OMP2-expressing zone. Scale bar, 50 μm