Fig. 3From: Massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of Bacillus cereus enterotoxin operons hbl, cytK and nhe Correlation of pairwise distance of concatenated housekeeping genes with whole genome ANI values. a: Correlation of whole-genome ANI and pairwise distance of seven concatenated housekeeping genes of all 142 B. cereus sensu lato strains. Intra-cluster values (for clusters see Fig. 1) are depicted in black, all inter-cluster values in grey. Pearson’s correlation of all values is r2 = 0.974. b and c: Intra-cluster comparison of whole-genome ANI and pairwise distance of seven concatenated housekeeping. Cluster affiliation (I – VII) according to species tree (Fig. 1). Strains that are difficult to sort into a distinct genomospecies are named and marked by circles. b: Clusters I, IV – VI can be distinguished at an ANI species boundary of ≥ 94 %. Cluster I: ANI 98.3 %, pairwise distance 0.005 substitutions per site. Cluster VII: ANI 99.5 %, pairwise distance 0.001 substitutions per site. c: Clusters II and III cannot be discerned on the basis of the comparisons presented: strain B. cereus F528/94 (#242) is affiliated to Cluster III by MLSA (Fig. 1) and whole-genome SNP analysis (Fig. 2), but to Cluster II by ANI (Additional file 2: Figure S1)Back to article page