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Table 3 The effects of fish group (including the effects of sex and ploidy associated with reproduction mode) and season on immunity parameters and steroid hormone levels

From: Physiological and condition-related traits in the gynogenetic-sexual Carassius auratus complex: different investments promoting the coexistence of two reproductive forms?

Dependent variable

Predicted variables

SS

Df

F

p

Total F

p

IgM

body size

0.032

1

0.002

0.962

8.981

<0.001

(N = 155)

season

261.658

3

6.224

<0.001

  
 

fish group

615.606

2

21.965

<0.001

  
 

fish group*season

415.222

6

4.938

<0.001

  

Oxidative burst (integral)

body size

0.038

1

2.343

0.128

13.611

<0.001

(N = 148)

season

1.996

3

40.764

<0.001

  
 

fish group

0.012

2

0.382

0.683

  
 

fish group*season

0.110

6

1.120

0.354

  

Oxidative burst (peak)

body size

0.272

1

4.703

0.032

10.272

<0.001

(N = 152)

season

4.995

3

28.742

<0.001

  
 

fish group

0.060

2

0.522

0.595

  
 

fish group*season

0.717

6

2.062

0.062

  

Lysozyme concentration

body size

0.135

1

0.572

0.451

6.322

<0.001

(N = 132)

season

8.558

3

12.086

<0.001

  
 

fish group

0.704

2

1.492

0.229

  
 

fish group*season

1.955

6

1.380

0.228

  

Complement activity

body size

0.010

1

1.139

0.288

14.384

<0.001

(N = 135)

season

1.287

3

48.703

<0.001

  
 

fish group

0.021

2

1.183

0.310

  
 

fish group*season

0.197

6

3.730

0.002

  

Cortisol level

body size

0.189

1

2.955

0.089

5.363

<0.001

(N = 108)

season

2.801

3

14.630

<0.001

  
 

fish group

0.312

2

2.446

0.092

  
 

fish group*season

0.332

6

0.866

0.523

  

Estradiol level

body size

0.000

1

0.000

0.984

40.453

<0.001

(N = 88)

season

10.453

3

51.065

<0.001

  
 

fish group

2.040

2

14.950

<0.001

  
 

fish group*season

6.691

6

16.342

<0.001

  

11-ketotestosterone level

body size

0.125

1

1.131

0.295

27.994

<0.001

(N = 43)

season

5.144

3

15.477

<0.001

  
 

fish group

17.156

2

77.423

<0.001

  
  1. The statistically significant p-values are shown in bold