Fig. 7

Comparison of cAMNs and iAMNs in WLG2 of the investigated pycnogonids. SLI signal shown in inverted b/w images for better contrast. In order to reveal as many details of the neurites as possible, clipping planes have been applied to Imaris volumes. Dashed lines indicate midline region. Red asterisks highlight cAMN somata, red arrowheads (numbered) illustrate the course of the primary neurite of the cAMN of one body half. Corresponding light blue markings have been used of the iAMNs. a: P. litorale. The soma of the right cAMN is in an unusually far lateral position. The dashed white rectangle highlights the lateral extensions that interconnect the SL-ir median domain with the more lateral SL-ir domains in each hemiganglion. b: Meridionale sp. Note the weakly stained somata of additional iAMNs. c: C. japonicus. Owing to the dense neuropilar meshwork in the median domain it is challenging to follow the delicate contralateral neurite of the cAMN (see also Additional file 4)