Fig. 3

Peptide alignment of D. melanogaster, An. gambiae, Cx. quinquefasciatus and Ae. aegypti doublesex genes; divided into 1. Common region (present in both male and female peptide), 2. Female-specific region 1 (C-terminus of female-specific protein), 3. Female-specific peptide C-terminus generated by use of alternate exon 4 splice donor, and 4. Male-specific region (C-terminus of male-specific protein). NCBI identification numbers are appended to the sequence ID. The in-frame intronic sequence is in bold/underline. The DNA-binding oligomerization domain (DBD/OD1) is boxed in yellow, while the common and female-specific portions of OD2 are boxed in orange