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Table 1 PCR cycling parameters for all DNA regions included in this study

From: Phylogeny and classification of the East Asian Amitostigma alliance (Orchidaceae: Orchideae) based on six DNA markers

DNA marker nrITS matK trnL-F trnS-trnG psbA-trnH Xdh
Initialization 94 °C 240 s 94 °C 240 s 94 °C 240 s 94 °C 240 s 94 °C 240 s cf. Górniak et al. [48]
Denaturation 94 °C 40–50 s 94 °C 45 s 94 °C 40–50 s 94 °C 45 s 94 °C 45 s
Annealinga 55 °C 40–50 s 48 °C 40–50 s 55 °C 40–50 s 52 °C 50–60 s 53 °C 50 s
Extension 72 °C 50–60 s 72 °C 60 s 72 °C 50–60 s 72 °C 50–60 s 72 °C 50–60 s
Number of cycles 30 30 30 30 30
Final extension 72 °C 420 s 72 °C 420 s 72 °C 420 s 72 °C 420 s 72 °C 420 s
  1. aInitially, the annealing temperatures for all regions were set to 53 °C; for samples with low yield, the temperatures were then adjusted to those specific for each region