Fig. 2

Relative MCFA content in mutant tgl3Δ and loa1Δ strains. Growth tests on solid YP medium at 23 °C and 28 °C were performed on loa1Δ cells (A). Relative amount of MCFAs (C8:0 to C14:0) in the wild-type (WT), tgl3Δ and loa1Δ grown at 23 °C or 28 °C in complete medium (YP) was determined using gas chromatography (B). Lipids from wild-type (WT) and mutant cells grown at 23 °C in YP medium were extracted using the Folch method. Polar and nonpolar lipids were then separated using solid-phase extraction. Total lipids and polar and nonpolar fractions were analyzed using gas chromatography (C). FAME, fatty acid methyl ester. Significant difference according to Student’s t-test, ***P < 0.001