Fig. 1

Relative MCFA content in wild-type S. cerevisiae. Relative amount of MCFAs (C8:0 to C14:0) in BY 4741 grown at 23 °C or 28 °C in complete medium (YP) or synthetic media (YNB) with high or low nitrogen was determined using gas chromatography (A). Lipids from cells grown at 23 °C in YP medium were extracted using the Folch method. Polar and nonpolar lipids were then separated using solid-phase extraction. Total lipids and polar and nonpolar fractions were analyzed using thin layer chromatography (B) and gas chromatography (C). FAME, fatty acid methyl ester; SE, sterol ester; TAG, triacylglycerol; S, sterol; PL, phospholipids. Significant difference according to Student’s t-test, ***P < 0.001, **P < 0.01, *P < 0.05