ML tree of the GlyRS homodimer subunit. Branch colors depict Bacteria (blue), Euryarchaeota (red), Crenarchaeota (green) and Eukarya (magenta). More narrow taxonomic designations are bracketed and labeled. Dotted lines represent branches with <50% BS support; major clades with >80% BS support are labeled with respective BS support values. Italic numbers represent posterior probability (PP) support values from tree reconstruction in PB. The GlyRS homodimer alignment contains 199 taxa, an alignment length of 1344 amino acids and rooted using sequences from ProRS and SerRS of Escherichia coli and Methanosarcina acetivorans (not shown). For the PB reconstruction, a subset of the alignment was used, consisting of 400 well-aligned sites as identified by GUIDANCE. Branch lengths depict substitutions/site. (**)PP support for the placement of the root was not calculated under PB, which failed to converge when outgroups were included. Non-converged PB runs including outgroups placed the outgroup along the same branch as the ML tree.