Figure 1

Multiple sequence alignment of AtMDLs, HsMIF and HsDDT. Amino acid sequences of AtMDL1 (AAO42959), AtMDL2 (NP_195785), AtMDL3 (AEE78824), HsMIF (P14174) and HsDDT (P30046) were aligned with ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/) using standard parameters. Subsequently, the alignment was shaded with BoxShade 3.21 (http://www.ch.embnet.org/software/BOX_form.html) using default settings. A black shade indicates identical amino acids; a grey shade denotes similar amino acids. In the consensus line, asterisks specify invariant amino acids and dots conserved amino acids with similar biophysical properties at a given position. The black triangle highlights the relative position of an intron found in all five genes, the white triangle the relative position of an intron present in AtMDL1 and AtMDL2 and the grey triangle the relative position of an intron found in HsMIF and HsDDT. The white arrows denote the position of C⁵⁷ and C⁶⁰ in HsMIF, the black bar above part of the sequence indicates the location of InterProScan domain IPR019829 (MIF, conserved site) in HsMIF and HsDDT, which overlaps with the TPOR motif. The black arrows point at residues R¹² and D⁴⁵ in HsMIF that form the pseudo-ELR motif and the grey bar above part of the sequence signifies the position of the predicted NES in AtMDL1 and HsMIF.