Genes regulated by acRPB1 are significantly enriched for evolutionary origins from early eukaryotes through early animals. ProteinHistorian analysis of the evolutionary origins of acRPB1 enriched genes (A) and acRPB1 dysregulated genes (B) compared with relevant background gene sets. Each bar gives the difference between the proportion of genes of interest and background genes originating in the last common ancestor of a given taxon (* = p < 0.05, ** = p < 0.01, *** = p < 0.001, all Bonferroni corrected). The proportion of genes of interest with origins in each taxon is given along the x-axis. For example, 18% of the acRPB1 enriched genes likely appeared between the origin of Opisthokonta and Bilateria, and this is significantly more (~7%, p < 0.001) than expected from the background of all genes with occupancy data.