Skip to main content

Table 1 Characteristics of isoforms of four enzymatic antioxidants in Symbiodinium

From: Transcriptomic characterization of the enzymatic antioxidants FeSOD, MnSOD, APX and KatG in the dinoflagellate genus Symbiodinium

Isoform

# of sequences

Identical sites

Clades represented

# of sequences with N-termini

Subcellular location*

TargetP reliability class [PLANT]

TargetP reliability class [NON-PLANT]

# of full length sequences

Ungapped length [aa]

Predicted molecular weight [kDa]

Predicted GPI-anchor [GPI-SOM/PredGPI]

SymMnSOD1

6

89.1%

B, C

5

M/S1

M4-5; S3

M4-5; S3-5

4

263-265

28.6-28.9

No/No

SymMnSOD2

4

84.9%

B, D, F

4

M/S2

M5; S5

M3-5; S3

4

294-303

32.0-32.6

No/No

SymMnSOD3

5

55.5% (73.3%3)

A, B, D

3

S

S2-3

S1-2

2

246-247

26.5-27.2

No/No

SymMnSOD (others)

6

 

A, B

5

S

S2-3

S1-3

1

270

29.2

No/No

SymFeSOD

4

92.7%

A, B, E, F

1

C

-

-

1

201

21.9

No/No

SymAPX1

23

26.1%

A, B, C, D, F

14

C

-

-

6

391-397

42.4-43.6

No/No

SymAPX2

7

67.1%

A, B, C, D

2

M4/S5

M5; S1

M4; S1

2

311/362

33.9/38.6

No/No

SymAPX3

6

69.5%

A, D

5

C

-

-

4

317-320

34.7-34.9

No/No

SymAPX4

3

87.3%

B, D, F

1

C

-

-

1

308

33.6

No/No

SymAPX5

3

86.1%

A, D

2

C

-

-

2

453

49.0/49.1

No/No

SymAPX6

2

69.2%

A, B

1

M

M5

S5

1

328

36.0

Yes7/No

SymAPX (others)

2

 

A

2

?

Not recognized

Not recognized

0

-

-

 

SymKatG1

12

63.6%

A, B, C, D, E, F

5

S

S2-5

S1-S5

1

486

54.4

Uncertain7/No

SymKatG2

4

73.1%

A, B, D

3

M6/S

M4; S5

S2-3

1

423

46.7

No/No

  1. Molecular characterization of superoxide dismutase (FeSOD/MnSOD), ascorbate peroxidase (APX), and catalase peroxidase (KatG) protein isoforms identified in the genus Symbiodinium. Proportion of identical residues across represented clades is indicated for each isoform (for pairwise identities between types see Additional files 1, 5 and 7). Subcellular location indicated as cytosolic (C), mitochondrial (M), or as part of the secretory pathway (S). TargetP reliability classes (1-5) are indicated, where 1 indicates the strongest prediction. Length, molecular weights, and presence of GPI anchor are based on full-length sequences.
  2. *based on results with “PLANT” settings in TargetP 1.1. for all sequences with complete N-terminal domains.
  3. weights for SOD metalloforms and KatG refer to the monomer.
  4. 1only Symbiodinium C15 M. digitata SymMnSOD1.
  5. 2only Symbiodinium B1 Mf1.05b SymMnSOD2 rep_c13368 and Symbiodinium of clade D A. hyacinthus SymMnSOD2 [GenBank:GAFP01017905].
  6. 3including Symbiodinium A1 CCMP2467 SymMnSOD3 Assembly2 sequence.
  7. 4 Symbiodinium of clade D A. hyacinthus SymAPX2 [GenBank:GAFP01018157].
  8. 5 Symbiodinium of clade D A. hyacinthus SymAPX2 [GenBank:GAFP01007188].
  9. 6 Symbiodinium of clade D A. hyacinthus SymKatG2 [GenBank:GAFP01010883].
  10. 7GPI-SOM results based on C&N-terminal signal.