Table 1 Primers (5′ → 3′) used to amplify and sequence major histocompatibility class II genes in H. abdominalis
# | Name | Sequence 5′-3′ | Location |
|---|---|---|---|
1 | MHIIα-E1F3 | TTACTCCGTTGCGGCGACGCC | Exon 1 |
2 | MHIIα-I1R | CGTCTGTACAATACTTCCGTAC | Intron 1 |
3 | MHIIα-I1F | CAGTTACCAGGACAATGAC | Intron 1 |
4 | MHIIα-I1F2 | CAAAAAGCGGTGCTTATCGAG | Intron 1 |
5 | MHIIα-I1R2 | GCTTTAACTTGAGATACAAGTACC | Intron 1 |
6 | MHIIα-I1R3 | CACCAACAGTGAAAAACCACAAG | Intron 1 |
7 | MHIIα-I1F3 | CTTGCGGGGGAAACGGCGAAG | Intron 1 |
8 | MHIIα-E2F* | GACGTCATCCACACAGACATGC | Exon 2 |
9 | MHIIα-E2R2 | GATTCTAGTGGCTGATCCTTCAATGC | Exon 2 |
10 | MHIIα-E3R* | TGATGGGATGGGAGGGAGGATC | Exon 3 |
11 | MHIIα-E3F | AGACCTTCCAACTGGACTTCAC | Exon 3 |
12 | MHIIα-E3R2 | GTGAAGTCCAGTTGGAAGGTCTGG | Exon 3 |
13 | MHIIα-I3R | CACGTTATTTATCATCGGCATAG | Intron 3 |
14 | MHIIα-E4F | CATCAAAGGAAACCAATGCAACTG | Exon 4 |
15 | MHIIα-3UTRR | GTGAGGACATAATGTGCGCCACC | 3′ UTR |
16 | MHIIα-3UTRF | GGTGGCGCACATTATGTCCTCAC | 3′ UTR |
17 | MHIIβ-5UTRR5 | GCAATACACTGGGGTTCTCTG | 5′ UTR |
18 | MHIIβ-5UTRF2 | CAGTGTATTGCAGAACATGG | 5′ UTR |
19 | MHIIβ-5UTRR6 | TATCGGTACTGGCCCATCC | 5′ UTR |
20 | MHIIβ-5UTRF | GCGACCAAAATCCGTTCCAG | 5′ UTR |
21 | MHIIβ-5UTRR4 | GGCGTGTTTGTTGTGATTTACAC | 5′ UTR |
22 | MHIIβ-5UTRF3 | GCTTTGTGCAACTAATTGTGC | 5′ UTR |
23 | MHIIβ-5UTRR2 | GGATCCAGTCTAATCTGAATCCCG | 5′ UTR |
24 | MHIIβ-5UTRR | CCAACCTGAGCACAAACTTG | 5′ UTR |
25 | MHIIβ-UTR5F | CAAGTTTGTGCTCAGGTTGG | 5′ UTR |
26 | MHIIβ-E1R | AAGGTGAGGAAAAGGAGGC | Exon 1 |
27 | MHIIβ-E1F2* | GCCTCCTTTTCCTCACCTTC | Exon 1 |
28 | MHIIβ-E2R2 | GATGTCATTCTGGTCACTCGAGTT | Exon 2 |
29 | MHIIβ-E2R* | GAGCGCACTTTCGTAGTCAA | Exon 2 |