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Figure 4 | BMC Evolutionary Biology

Figure 4

From: Empirical demonstration of environmental sensing in catalytic RNA: evolution of interpretive behavior at the origins of life

Figure 4

Semi-formal kinetic analysis of ribozyme activities under the four environmental conditions O1–O4 using 5′- 32P-radiolabeled substrate-cleavage assays. (A) graph of percent substrate reacted as a function of time for the CG enzyme at two different concentrations: 25 nM (solid lines) and 10 nm (dotted lines). Horizontal dashed line indicates 5 min time point, where pick-up-the-tail data for interpretive analysis (Figure 3) was selected. (B) Example radiogram of cleavage patterns in the case of the CG ribozyme at 25 mM and *S-1t at 1 nM.

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