Table 2 Primers and annealing temperatures for PCR amplification
From: Ancient DNA provides new insight into the maternal lineages and domestication of Chinese donkeys
MtDNA | Primers | Sequence(5’-3’) | Annealing temperature | Length | |
|---|---|---|---|---|---|
D-loop | 1F/1R | L15424 | CACCATCAACACCCAAAGCT | 50.8°C | 201 bp |
H15625 | ACATGCTTATTATTCATGGGGC | ||||
6F/6R | L15599 | GCCCCATGAATAATAAGCA | 51.5°C | 257 bp | |
H15855 | TGAAGAAAGAACCAGATGCC | ||||
4F/5R | L15828 | TGAAACTATACCTGGCATCTGG | 51.5°C | 280 bp | |
H16107 | CATGGACTGAATAACACCTTATGG | ||||
Cytb | 7F/7R | L14936 | GAGACCCAGACAACTACACC | 50.5°C | 235 bp |
H15170 | ACTAAGAGTCAGAACACGCA | ||||
8F/8R | L15133 | TTCCGACCCCTTAGTCAATG | 52.6°C | 251 bp | |
H15383 | AATGTTTCCCCCTTTTCTGG | ||||