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Table 2 AMOVA of intra- and interspecific cultivar variation

From: Variable interaction specificity and symbiont performance in Panamanian Trachymyrmex and Sericomyrmexfungus-growing ants

Source of variation Degrees of freedom Sum of squares Variance component explained variation (%) Fixation index P value
Complete dataset       
Between genera 1 640.97 21.64 27 ΦCT =0.27 0.3292
Among species within genera 4 774.25 31.87 39 ΦST =0.66 < 0.0001
Within species 32 876.26 27.38 34 ΦSC =0.54 < 0.0001
Total 37 2291.48 80.89 100   
T. cornetzi sp. 1–3 and T. zeteki       
Among species within genera 3 330.22 12.18 17 ΦST =0.17 0.0645
Within species* 15 875.36 58.35 83   
Total 18 1205.58 70.53 100   
  1. Results of AMOVA of Leucoagaricus symbiont diversity of the full dataset with three hierarchical levels and the second analysis considering only the T. cornetzi and T. zeteki species whose fungal symbionts were variable enough in their ITS sequences to represent different species (Figure 1). Significances are based on 10100 permutations evaluating whether fixation indices were different from a null distribution of variance parameters assuming samples were drawn from randomly chosen species.
  2. *Only a single null distribution, assuming samples randomly drawn from within species, was generated, which precluded a permutation-based significance test for this level in the two-level pairwise AMOVA.