High lifetime inbreeding depression counteracts the reproductive assurance benefit of selfing in a mass-flowering shrub

Table 1 Experimental manipulation for estimating pollination parameters: reproductive assurance (RA) and self-compatibility (SC) estimated with seed sets from four different pollination treatments ( IN , EN , IX and ISB )

Abbreviation and calculation	Definitions	Patch mean seed sets/indices	Range	Tukey-Kramer test
*Reproductive assurance (RA)*
IN	Seed set from intact inflorescences allowed to undergo natural pollination	0.68	0.46 - 0.9	IN vs. EN t =7.86; P <0.0001
N =28 patches		0.68	0.46 - 0.9
EN	Seed set from emasculated inflorescences allowed to undergo natural pollination	0.49	0.13 - 0.79
N =27 patches		0.49	0.13 - 0.79
RA =1 – ( *ENm* / *INm* )	Proportion of seed production attributable to autogamous self-pollination (including autonomous and facilitated)	0.27	−0.15 - 0.86
N =27 patches		0.27	−0.15 - 0.86
*Self-compatibility (SC)*
IX	Seed set from intact inflorescences outcrossed by hand	0.71	0.40 - 1	IX vs. ISB t = − 9.04; P <0.001
N =28 patches	Seed set from intact inflorescences outcrossed by hand	0.71	0.40 - 1
*ISB*	Seed set from bagged intact inflorescences self-pollinated by hand	0.5	0.31 - 0.80
N =28 patches		0.5	0.31 - 0.80
SC = *ISBm* / IX m	Early-acting ID or partial self-incompatibility effect on seed set in selfed flowers	0.71	0.53 - 1
N =28 patches		0.71	0.53 - 1

Patch means, ranges and Tukey-Kramer tests are presented to compare IN vs. EN (reproductive assurance) and IX vs. ISB (self-compatibility). See the Method section for model description and Additional file 1: Table S1 for raw data per patch.

ISSN: 2730-7182