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Table 1 Experimental manipulation for estimating pollination parameters: reproductive assurance (RA) and self-compatibility (SC) estimated with seed sets from four different pollination treatments ( IN , EN , IX and ISB )

From: High lifetime inbreeding depression counteracts the reproductive assurance benefit of selfing in a mass-flowering shrub

Abbreviation and calculation Definitions Patch mean seed sets/indices Range Tukey-Kramer test
Reproductive assurance (RA)
IN Seed set from intact inflorescences allowed to undergo natural pollination 0.68 0.46 - 0.9 IN vs. EN t =7.86; P <0.0001
N =28 patches
EN Seed set from emasculated inflorescences allowed to undergo natural pollination 0.49 0.13 - 0.79
N =27 patches
RA =1 – ( ENm / INm ) Proportion of seed production attributable to autogamous self-pollination (including autonomous and facilitated) 0.27 −0.15 - 0.86  
N =27 patches
Self-compatibility (SC)
IX Seed set from intact inflorescences outcrossed by hand 0.71 0.40 - 1 IX vs. ISB t = − 9.04; P <0.001
N =28 patches
ISB Seed set from bagged intact inflorescences self-pollinated by hand 0.5 0.31 - 0.80
N =28 patches
SC =ISBm / IX m Early-acting ID or partial self-incompatibility effect on seed set in selfed flowers 0.71 0.53 - 1  
N =28 patches
  1. Patch means, ranges and Tukey-Kramer tests are presented to compare IN vs. EN (reproductive assurance) and IX vs. ISB (self-compatibility). See the Method section for model description and Additional file 1: Table S1 for raw data per patch.