Figure 3

Neighbor joining tree of unique Rsp sequences in D. melanogaster and Rsp-like sequences in species of the simulans clade with D. yakuba as the outgroup suggests that Rsp evolves rapidly in D. melanogaster . These unique sequences were compiled from the NGS reads in each species. The Rsp-like sequences of D.simulans (red), D. sechellia (blue) and D. mauritiana (orange) are interleaved throughout the tree whereas the canonical Rsp in D. melanogaster (green) forms its own clade.