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Table 2 CA expression patterns of CAs in S. ciliatum

From: Calcareous sponge genomes reveal complex evolution of α-carbonic anhydrases and two key biomineralization enzymes

CA

ISH

RNA-Seq

SciCA1 (scl-CA1)

Sclerocytes, weak signal in ring of posterior-most micromeres in larvae

All stages (including spicule-free larva and postlarval stage i), higher expression in top region of sponge

SciCA2 (scl-CA2)

Sclerocytes, weak signal in ring of posterior-most micromeres in larvae

All stages (including spicule-free larva and postlarval stage i), higher expression in top region of sponge

SciCA3

Elevated expression in oocytes and early embryos; ubiquitous expression in other cell types including all larval cells.

All stages, higher expression in sponges with oocytes and early embryos, larvae and postlarval stage i

SciCA4

Larval macromeres

In larvae and postlarval stages ii-v, low expression in adult sponges

SciCA5

Undetected

Higher in larvae, postlarval stages i-iii, young syconoid sponge, low in other stages and adult sponges

SciCA6

Undetected

Very low expression during embryonic and postembryonic development

SciCA7

Proximal exopinacocytes between radial tubes

Low expression in adult sponges

SciCA8

Undetected

Low expression in adult sponges

SciCA9 (LCA)

Undetected

Highly expressed in postlarval-juvenile stages i-v, low expression otherwise