Influence of the Pedinophyceae on the placement of the Chlorellales. (A) Phylogeny of chlorophytes inferred under the GTR + Γ4 model using the amino acid data set of 15,549 positions after exclusion of the Pedinophyceae. The best-scoring RAxML tree is presented and support values are reported on the nodes, with black dots indicating 100% BS values. The scale bar denotes the estimated number of amino acid substitutions per site. (B) Confidence assessment of the three possible topologies (T1, T2 and T3) for the placement of the Chlorellales under the GTR + Γ4 model. The ΔlnL value indicates the difference in log likelihood relative to the best tree (T1). Local bootstrap probabilities were estimated by resampling of the estimated log-likelihood (RELL). pAU, P value for the approximately unbiased (AU) test  as implemented in CONSEL 0.20 .