Table 1 Primers for immune sequences of Nicrophorus vespilloides
From: Egg survival is reduced by grave-soil microbes in the carrion beetle, Nicrophorus vespilloides
Gene | Forward primer | Reverse primer |
|---|---|---|
Attacin2 | 5’-ACGTCACAGGAGAAGAGCTGA-3’ | 5’-TCGGAAGGCCTGTGTGTGTA-3’ |
Defensin1 | 5’-GTCGATACGCCCATCGGTTC-3’ | 5’-GCAATTGCAGACTCCGTCGA-3’ |
Defensin2 | 5’-AGAGGTGCATGCGATCTGTT-3’ | 5’-TGTGCCTTTGGTGTATCCGT-3’ |
Coleoptericin1 | 5’-CGAAACGGTGGTGAACAGGT-3’ | 5’-TGCATTGGTTGTACCGTCGG-3’ |
Coleoptericin2 | 5’-TGGTCTCCGCCGAATCCTAA-3’ | 5’-GCACCTGGTCTTTCGTGCTT-3’ |
Coleoptericin3 | 5’-ACTTTGGCGCGAGTCGATTT-3’ | 5’-TTGATCGCCCAACTCGCTTC-3’ |
RPL7 | 5’-TGCCATCAAGAAGCGCTCTG-3’ | 5’-GCGCTCTTGGCTTGATGGAT-3’ |