Strong genetic structure corresponds to small-scale geographic breaks in the Australian alpine grasshopper Kosciuscola tristis

Table 1 Genetic variation in markers used for phylogeny reconstruction ( CO1 and ITS1 ) and population genetic analyses

	CO1		ITS1	Microsatellites (five shared loci)			Microsatellites (all typed loci)
Location	Nucleotide diversity (π)	Number of haplotypes	Number of haplotypes	H _O	H _E	N _a	H _O	H _E	N _a
Kosciuszko region
Mt Jagungal	0.001	2	1	0.61	0.83	11.80	0.56	0.83	11.63
Mt Tate	0.002	4	1	0.51	0.81	12.40	0.51	0.82	11.88
Guthega	0.002	4	3	0.50	0.81	13.60	0.48	0.82	12.00
Mt Townsend	0.001	3	1
Thredbo 1	0.001	2	1	0.56	0.82	12.40	0.53	0.79	10.25
Thredbo 2				0.51	0.83	13.20	0.48	0.80	11.25
Thredbo 3				0.57	0.81	13.80	0.50	0.77	11.25
Victoria
Mt Bogong	0.008	2	1	0.46	0.88	13.40
Mt Nelse	0.001	4	2
Falls Creek	0.000	1	1	0.51	0.90	14.20
Mt Cope	0.000	1	1	0.38	0.87	12.20
Mt Jaithmathang	0.002	2	1
Mt Hotham	0.041	5	2
Mt Buffalo	0.002	5	1	0.26	0.80	9.80
Mt Buller	0.001	2	1	0.49	0.78	8.80
Mt Stirling	0.000	1	1	0.33	0.69	10.00
Mt Baw Baw	0.002	3	5	0.27	0.59	7.40

Genetic variation was measured as nucleotide diversity and haplotype number for CO1; nucleotide diversity was not calculated for ITS1 as variation was very low. Observed heterozygosity (Ho), expected heterozygosity (HE) and mean allelic richness (Na) were calculated for five microsatellite loci that amplified across all populations, as well as for eight loci typed for the Kosciuszko populations.

ISSN: 2730-7182