New subfamilies of major intrinsic proteins in fungi suggest novel transport properties in fungal channels: implications for the host-fungal interactions

Table 1 Aromatic/arginine selectivity filter residues and substitutions in the conserved NPA motifs in different fungal MIP subgroups

		Aromatic/arginine selectivity filter^c
Fungal MIP subgroup^a	Number of sequences/Total number of sequences^b	TM2	TM5	LE1	LE2	NPA substitution (%)^d
FPS-1 like AQGPs	25/26	W	L/I/M/N	T/C/A/S	R	61.7
Facultative	17/42	W	A/S/T/G/D	G/A	R	66.7
(Yfl054-like)	8/42	W	V/I	G	R
AQGPs	5/42	W	M	L	R
	8/42	W	G	F/Y	R
α-cluster AQGPs	46/48	W	G	Y/W/F	R	95.8
β-cluster AQGPs	20/24	W	G	Y	R	12.5
γ1-cluster AQGPs	4/5	T/N/S	C/T	F	R	20
γ2-cluster AQGPs	5/5	W/M/I	G	Y	R	0
δ-cluster AQGPs	49/49	F/Y/M	V/I/A	I/L/V	R	100
AQPs	76/163	F/M/A	H	T/A/C/S	R
	64/163	F/I/A	E	G	R	28.8
	15/163	Y/F	M/L/V/A	A/G	R
	8/163	F/M	Q	C/G	R
XIPs	17/17	N/S/A	S/A/G	A/F	R/K	82.4
SIP-like	16/16	V/I/L/M	A/S/G	G	L/I	81.2

^aFor details of different fungal MIP subgroups, see Figure 1 and the text.
^bNumber of sequences possessing a particular type of Ar/R selectivity filter residues and the total number of sequences belonging to that particular fugal MIP subgroup are given.
^cThe four residues forming the selectivity filter of an MIP channel are contributed by the second (TM2) and fifth (TM5) transmembrane segments and two residues from the loop E region (LE1 and LE2).
^dPercentage of sequences from that particular fungal MIP subgroup that has substitution(s) in at least one of the conserved NPA motifs.

ISSN: 2730-7182