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Table 3 Analysis of functional divergence between OPR subfamilies in plants

From: Phylogenetic analysis, structural evolution and functional divergence of the 12-oxo-phytodienoate acid reductase gene family in plants

Comparison θ1 ± S.Ea P b Qk>0.70c Critical amino acid sitesd
Sub. I vs II 0.220 ± 0.056 <0.01 7 35*, 65, 70*, 110, 258*, 306, 324*
Sub. I vs III 0.280 ± 0.055 <0.01 11 41*, 54, 69*, 80, 104, 243*, 258*, 312, 324*, 330*, 360
Sub. I vs IV 0.176 ± 0.070 <0.01 1 212
Sub. I vs V 0.351 ± 0.083 <0.01 7 62*, 98, 166, 212, 227*, 254*, 264
Sub. I vs VI 0.229 ± 0.063 <0.01 5 47, 110, 157*, 212, 307*
Sub. I vs VII 0.377 ± 0.077 <0.01 16 38, 43, 56*, 62*, 68, 85, 163*, 178, 207*, 227*, 245, 257,258*, 327*, 331, 332
Sub. II vs III 0.340 ± 0.064 <0.01 14 41*, 65, 69*, 70*, 80, 85, 167, 169, 194, 201, 243*, 306, 308, 309
Sub. II vs IV 0.001 ± 0.022 >0.05 0 Not found
Sub. II vs V 0.142 ± 0.133 >0.05 0 Not found
Sub. II vs VI 0.269 ± 0.064 <0.01 10 38, 65, 157*, 191, 223, 245, 248*, 307*, 308, 324*
Sub. II vs VII 0.114 ± 0.083 <0.05 0 Not found
Sub. III vs IV 0.350 ± 0.086 <0.01 8 21, 40*, 83*, 187, 254*, 307*, 308, 309
Sub. III vs V 0.437 ± 0.084 <0.01   16 *62, 66, 70*, 82, 104, 158*, 176, 243*, 254*, 255, 258*, 264, 273, 275, 318, 330*
Sub. III vs VI 0.353 ± 0.068 <0.01 13 21, 70*, 87, 158*, 243*, 248*, 255, 259, 309, 312, 314*, 318, 324*
Sub. III vs VII 0.378 ± 0.081 <0.01 12 56*, 62*, 68, 69*, 85, 189, 205*, 228*, 233, 245, 324*, 330*
Sub. IV vs V 0.288 ± 0.155 <0.05 0 Not found
Sub. IV vs VI 0.351 ± 0.083 <0.01 9 47, 157*, 189, 223, 248*, 254*, 265, 307*, 308
Sub. IV vs VII 0.259 ± 0.153 <0.05 0 Not found
Sub. V vs VI 0.159 ± 0.105 >0.05 0 Not found
Sub. V vs VII 0.316 ± 0.162 <0.05 2 264, 332
Sub. VI vs VII 0.178 ± 0.072 <0.01 1 245
  1. a θ is the coefficient of functional divergence; θ1 ± S.E. is the coefficient of type I functional divergence between two clusters and its standard error.
  2. b The significance level (P value) is computed using Fisher's transformation.
  3. c Qk, posterior probability. A site-specific profile based on the posterior probability (Qk) was used to identify critical amino acid residues that were responsible for functional divergence.
  4. d Numbering of amino acid residues corresponds to AtOPR02-1. Critical amino acid sites with the highest posterior values (Qk>0.70) are shown.
  5. * The amino acid residues depicted with an asterisk were also found to be predicted in positive-selection sites between OPR paralogues (see Table 2).