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Table 4 Primers to amplify the mitochondrial DNA of F. paralugubris

From: Genetic clusters and sex-biased gene flow in a unicolonial Formica ant

Locus

Forward primer

Reverse primer

Fragment size (bp)

COI-RLR

TTGATTTTTTGGTCATCCAGAAGT

TAGGTGAATTTGAATTTTGTAATG

~980

COI-II a

CGACGTTACTCCGAATACCC

TGGCCTTGAAGAAGAAATCG

~500

COI-II b

CAAAATTCAAATTCNCCNTATGA

CCNGGNGTTGAGTCTATTTT

~500

ND4–ND6 a

CAAATATGAAATAAATAAATTGG

GTTTGTGAAGGGGTTTTAGG

~760

ND4–ND6 b

AATTAAYAAAGTTAATCCTAAAACCCC

CGTATAGAGATAGATTTTATRGAACAG

~950

Cytb-Fe

CAGTTTAATTTCTAATGAACAAAC

GGATCTCTAAAAATATATGGG

~1030

ND6-ND1 a

ACATACCACAGGTTCATCAAATCC

CGAGGTTTATTACCTCGAATGCGTTATG

~810

ND6-ND1 b

AGTAACCCCAATCCATATTCAACC

AATAGGGTCTATGCGGTCAG

~1040

ND6-ND1 c

CATAACGCATTCGAGGTAATAAACCTCG

GTAGCATTTTTAACTTTATTAGAACG

~770

CbM3

TATTTTCCATTATTAAAATCTGTTC

AGTAAGAAATATTTACAGGTGAGGG

163/166

PB300 T/C

AAACTGACCGCATAGACCCTAT-T/C

GTGTTATGTTGATAAGGTGGG

171

PB30 A/G

CCCACCTTATCAACATAACAC

AATGCGGAAAGGTCCTAATAAGG-T/C

164

  1. The first nine pairs of primers listed above were used for the initial screen of three mitochondrial regions (COI-COII, ND4-ND6 and ND6-ND1). The last three pairs of primers (CbM3, PB300 T/C and PB30 A/G) were used for the routine typing of three polymorphic sites in the ND6-ND1 region.
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BMC Ecology and Evolution

ISSN: 2730-7182

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