Functional importance of residues in the different position of the alignment. Sequences were divided into two classes (CGFS and CPYC). Then, they were further divided into Archaeal, Bacterial and Eukaryotic sequences. Because archaea only have a small number of GRX proteins of the CGFS class, we show the results for archaea GRXs of the CPYC class only. Subsequently, Normalized Mutual Information (NMI) and Position Entropy were calculated for the different alignments. Vertical red lines in the plots of the first and second column indicate positions in the alignment that are highly conserved. Horizontal black lines in the plots of the first and second columns indicate positions in the alignment that have high NMI with at least one other position. Numbers in columns one and two indicate the position in the alignment. First column – CPYC class GRXs. Second column – CGFS class GRXs. Third and fourth columns – Detailed positions in the alignments that are colored, with the consensus residues found in those alignment positions. The putative active centers are shaded in blue. Average entropy per residue is also shown in columns three and four.