Relaxin gene family in teleosts: phylogeny, syntenic mapping, selective constraint, andexpression analysis

Table 3 Average pairwise dn/ds values of relaxin family genes in teleost and mammals.

Relaxin family Gene	B + A - chain	B-chain	A-chain
	d_n/d_s (d_s, d_n)	d_n/d_s (d_s, d_n)	d_n/d_s (d_s, d_n)
Teleost rln3a	0.08 (0.60, 0.05)	0.09 (0.57, 0.05)	0.07 (0.62, 0.04)
Teleost rln3b	0.07 (0.58, 0.04)	0.10 (0.57, 0.06)	0.05 (0.60, 0.03)
Teleost rln	0.09 (0.46, 0.04)	0.04 (0.45, 0.02)	0.11 (0.52, 0.06)
Teleost insl5a	0.25 (0.51, 0.13)	0.13 (0.53, 0.07)	0.20 (0.49, 0.10)
Teleost insl5b	0.40 (0.57, 0.23)	0.34 (0.58, 0.20)	0.48 (0.52, 0.25)
Teleost insl3	0.37 (0.63,0.23)	0.41 (0.64, 0.26)	0.45 (0.53, 0.24)
Mammalian RLN	0.64 (0.50, 0.32)	0.59 (0.56, 0.33)	0.60 (0.50, 0.30)
Mammalian RLN3	0.08 (0.51, 0.04)	0.04 (0.45, 0.02)	0.11 (0.56, 0.06)
Mammalian INSL3	0.36 (0.51, 0.18)	0.20 (0.50, 0.10)	0.40 (0.61, 0.24)
Mammalian INSL5	0.23 (0.79, 0.17)	0.19 (0.61, 0.12)	0.21 (0.98, 0.21)
Mammalian INSL6	0.46 (0.46, 0.20)	0.78 (0.32, 0.25)⁺	0.24 (0.58, 0.14)

Only the five teleost and four mammalian species for which complete genomic information was available were included in the analyses. Z-tests of whether d_n/d_s > 1 for any gene was performed using bootstrapping to compute the variance (+ p < 0.10, * p < 0.05). Average d_n/d_s < 0.1 represent strongly purifying selection, 0.11-0.5 represent weakly purifying seletion, and >1 represents recent positive selection. See text for details.

ISSN: 2730-7182