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Table 1 Primers designed for isolating and mapping BnFT paralogues.

From: The evolution of Brassica napus FLOWERING LOCUST paralogues in the context of inverted chromosomal duplication blocks

Primer name

Primer sequence

For amplifying BnFT probe from Tapidor genome

AtFT1

5'ACCCTCACCTCCGAGAATATCT3'

AtFT2

5'AGCCACTCTCCCTCTGACAAT3'

For isolating BnFT sequences from positive BACs

BrFT1

5'AGTTGTAGGAGACGTTCTTGA3'

BrFT2

5'AGATATTCTCGGAGGTGAGGAT3'

BrFT2-3

5'ATGGTGGATCCAGATGTTCCAA3'

BrFT4

5'AGCCACTCTCCCTCTGACAAT3'

BrFT1-5'

5'TTGGCCGTATGTAACCCTTAGATCGAT3'

BrFT4-3'

5'ACAATTCAACACTCGTGAGTTTGCT3'

For mapping BnFT paralogues

BnA2.FT-1

5'TCGAAGTGAGTAGGACTAGGT3'

BnA2.FT-2

5'TGCTCTAAGTGACTCCAAAACA3'

BnA7.FTa-1

5'CTCAGGTTCAAAACAAGCCAA 3'

BnA7.FTa-2

5'GATATTCTCGGAGGTGAGGGTT3'

BnA7.FTb-1

5'TGCATAGGAGACATCGAAGTT3'

BnA7.FTb-2

5'AGTTCTATAGGAGTGGACACA3'

BnC2.FT-1

5'ACCCTCACCTCCGAGAATATCT3'

BnC2.FT-2

5'AGCCACTCTCCCTCTGACAAT3'

BnC6.FTa-1

5'AGTAGGACCAGGTTTCCTGT3'

BnC6.FTa-2

5'TGGTATGGTGCATGCATACT3'

BnC6.FTb-1

5'ACGAGAATATCTCCATTGGT3'

BnC6.FTb-2

5'TCCATGTCTAGTTGTGT3'