Skip to main content
Figure 1 | BMC Evolutionary Biology

Figure 1

From: The evolution of Brassica napus FLOWERING LOCUST paralogues in the context of inverted chromosomal duplication blocks

Figure 1

Distribution of primers used to amplify the BnFT fragments, with arrows indicating direction. The top primer pairs (AtFT1 and AtFT2) were used to amplify the probe for screening BnFT paralogues from BAC clones of the JBnB library. Lower primers were used to amplify different regions of BnFT paralogues from positive BACs: BrFT1/BrFT2 for exon1-exon2, BrFT2-3/BrFT4 for exon2-exon4; 5' and 3' UTR were amplified with BrFT1-5' and BrFT4-3', respectively, by PCR walking.

Back to article page