Alignment of the C-terminal sequence of Daphnia pulex Spz7 and 8 to arthropod Spz2s. Alignment of the Cys knot sequence and C-terminal of one respective representative of Daphnia pulex Spz7 (Dappu-Spz7A) and Spz8 (Dappu-Spz8A) to Spz2 sequences of Daphnia (Dappu-Spz2), Aedes (Aa: Aedes aegypti) and Drosophila (Dm: Drosophila melanogaster). Conserved Cys forming the Cys knot are in white on black background and are numbered below from I to VI. Conserved residues between the sequences are indicated below by an asterisk (*). Arrowheads indicate Cys (also highlighted in grey) characteristically conserved in both Spz7 and Spz8, or only conserved in Spz8. The unique C-terminal extension of Spz2 proteins is present in Dappu-Spz2 as well as a conserved "NY(D/N)YHPIIDFF" sequence motif (in white on black) and these are absent in Spz7 and Spz8 representatives.